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Date: Sun, 28 Mar 2021 06:48:45 -0700
To: <b.smith@smithfamilycarpets.com>
Message-ID: <205138748144354205190410115982283@p76io7.proheros.biz>
From: "Clogged Arteries" <CloggedArteries@proheros.biz>
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Subject:    Cure High Cholesterol By Cutting Out This ONE Ingredient

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You're about to learn how cutting out ONE, single ingredient lowers your cholesterol level below 100 and clears out up to 93% clogged arteries – starting today!

Preventing diseases such as stroke and heart attack. Plus loading you with power and vigor.

Discover how to:
http://www.proheros.biz/2051-101-904-48144354/b.smith/tindex7.html
- Completely clean out the plaque buildup in your arteries
- Drop your cholesterol to a healthy level
- And boost your physical and mental energy to a level you didn’t think possible
...all by cutting out just ONE simple ingredient, you didn’t even know you were consuming.


Based on a little known secret, previously only available to the rich and famous.

What is this ONE ingredient you need to cut out? Learn more and try it out for yourself here...

 

 http://www.proheros.biz/2051-101-904-48144354/b.smith/tindex8.html
 

 
 

 

 

 




http://www.proheros.biz/2051-101-904-48144354/b.smith/tindex9.html

The study of proteins in vivo is often concerned with the synthesis and localization of the protein within the cell. Although many intracellular proteins are synthesized in the cytoplasm and membrane-bound or secreted proteins in the endoplasmic reticulum, the specifics of how proteins are targeted to specific organelles or cellular structures is often unclear. A useful technique for assessing cellular localization uses genetic engineering to express in a cell a fusion protein or chimera consisting of the natural protein of interest linked to a "reporter" such as green fluorescent protein (GFP).[68] The fused protein's position within the cell can be cleanly and efficiently visualized using microscopy,[69] as shown in the figure opposite. Other methods for elucidating the cellular location of proteins requires the use of known compartmental markers for regions such as the ER, the Golgi, lysosomes or vacuoles, mitochondria, chloroplasts, plasma membrane, etc. With the use of fluorescently tagged versions of these markers or of antibodies to known markers, it becomes much simpler to identify the localization of a protein of interest. For example, indirect immunofluorescence will allow for fluorescence colocalization and demonstration of location. Fluorescent dyes are used to label cellular compartments for a similar purpose.[70] Other possibilities exist, as well. For example, immunohistochemistry usually utilizes an antibody to one or more proteins of interest that are conjugated to enzymes yielding either luminescent or chromogenic signals that can be compared between samples, allowing for localization information. Another applicable technique is cofractionation in sucrose (or other material) gradients using isopycnic centrifugation.[71] While this technique does not prove colocalization of a compartment of known density and the protein of interest, it does increase the likelihood, and is more amenable to large-scale studies. Finally, the gold-standard method of cellular localization is immunoelectron microscopy. This technique also uses an antibody to the protein of interest, along with classical electron microscopy techniques. The sample is prepared for normal electron microscopic examination, and then treated with an antibody to the protein of interest that is conjugated to an extremely electro-dense material, usually gold. This allows for the localization of both ultrastructural details as well as the protein of interest.[72] Through another genetic engineering application known as site-directed mutagenesis, researchers can alter the protein sequence and hence its structure, cellular localization, and susceptibility to regulation. This technique even allows the incorporation of unnatural amino acids into proteins, using modified tRNAs,[73] and may allow the rational design of new proteins with novel properties


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<html>
<head>
=09<title>Newsletter</title>
</head>
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<div style=3D"width: 550px;font-family: Arial;font-size: 20px;padding: 15px=
;  font-size:18px;"><b style=3D"font-size:22px; color:#C70039 ; ">You&#39;r=
e about to learn how cutting out ONE, single ingredient lowers your cholest=
erol level below 100 and clears out up to 93% clogged arteries &ndash; star=
ting today!</b><br />
<br />
Preventing diseases such as stroke and heart attack. Plus loading you with =
power and vigor.<br />
<br />
<a href=3D"http://www.proheros.biz/2051-101-904-48144354/b.smith/tindex1.ht=
ml"><b>Discover how to:</b></a><br />
<br />
- Completely clean out the plaque buildup in your arteries<br />
- Drop your cholesterol to a healthy level<br />
- And boost your physical and mental energy to a level you didn&rsquo;t thi=
nk possible<br />
...all by cutting out just ONE simple ingredient, you didn&rsquo;t even kno=
w you were consuming.<br />
<br />
<br />
<b>Based on a little known secret,</b> previously only available to the ric=
h and famous.<br />
<br />
<a href=3D"http://www.proheros.biz/2051-101-904-48144354/b.smith/tindex2.ht=
ml"><b>What is this ONE ingredient you need to cut out? Learn more and try =
it out for yourself here...</b></a>
<p>&nbsp;</p>
</div>

<p>&nbsp;</p>
&nbsp;

<p>&nbsp;</p>
&nbsp;

<p>&nbsp;</p>

<p>&nbsp;</p>

<p>&nbsp;</p>
<br />
<br />
<br />
<br />
<a href=3D"http://www.proheros.biz/2051-101-904-48144354/b.smith/tindex3.ht=
ml" target=3D"blank"><img src=3D"http://www.proheros.biz/2051-101-904-48144=
354/i/img0101904387.jpg" /></a><br />
<br />
<p style=3D"color:#FFFFFF;font-size:6px;">The study of proteins in vivo is =
often concerned with the synthesis and localization of the protein within t=
he cell. Although many intracellular proteins are synthesized in the cytopl=
asm and membrane-bound or secreted proteins in the endoplasmic reticulum, t=
he specifics of how proteins are targeted to specific organelles or cellula=
r structures is often unclear. A useful technique for assessing cellular lo=
calization uses genetic engineering to express in a cell a fusion protein o=
r chimera consisting of the natural protein of interest linked to a "report=
er" such as green fluorescent protein (GFP).[68] The fused protein&#39;s po=
sition within the cell can be cleanly and efficiently visualized using micr=
oscopy,[69] as shown in the figure opposite. Other methods for elucidating =
the cellular location of proteins requires the use of known compartmental m=
arkers for regions such as the ER, the Golgi, lysosomes or vacuoles, mitoch=
ondria, chloroplasts, plasma membrane, etc. With the use of fluorescently t=
agged versions of these markers or of antibodies to known markers, it becom=
es much simpler to identify the localization of a protein of interest. For =
example, indirect immunofluorescence will allow for fluorescence colocaliza=
tion and demonstration of location. Fluorescent dyes are used to label cell=
ular compartments for a similar purpose.[70] Other possibilities exist, as =
well. For example, immunohistochemistry usually utilizes an antibody to one=
 or more proteins of interest that are conjugated to enzymes yielding eithe=
r luminescent or chromogenic signals that can be compared between samples, =
allowing for localization information. Another applicable technique is cofr=
actionation in sucrose (or other material) gradients using isopycnic centri=
fugation.[71] While this technique does not prove colocalization of a compa=
rtment of known density and the protein of interest, it does increase the l=
ikelihood, and is more amenable to large-scale studies. Finally, the gold-s=
tandard method of cellular localization is immunoelectron microscopy. This =
technique also uses an antibody to the protein of interest, along with clas=
sical electron microscopy techniques. The sample is prepared for normal ele=
ctron microscopic examination, and then treated with an antibody to the pro=
tein of interest that is conjugated to an extremely electro-dense material,=
 usually gold. This allows for the localization of both ultrastructural det=
ails as well as the protein of interest.[72] Through another genetic engine=
ering application known as site-directed mutagenesis, researchers can alter=
 the protein sequence and hence its structure, cellular localization, and s=
usceptibility to regulation. This technique even allows the incorporation o=
f unnatural amino acids into proteins, using modified tRNAs,[73] and may al=
low the rational design of new proteins with novel properties</p>

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